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<font size="10"> <img src="FinchScope/img/im1.png" width="50"/>FreedomScope Project</font>
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Overview
FreedomScope is an umbrella project for several customizable and scaleable single-photon fluorescent imaging microscope systems that take advantage of developing open-source landscape to provide a modular in-vivo all optical neurophysiology rigs with a transparent hardware, and acquisition and analysis software.
You can read more about the project here.
The project was developed by Will Liberti in the Gardner Lab, for multi-month imaging of neural activity in songbirds [1] [2] [3] bats, and rodents. This effort would not have been possible without the critical advice and support at the outset of the project by Daniel Aharoni and Peyman Golshani, of the UCLA Miniscope project
Components:
Finchscope Microscope
The FinchScope is a lightweight wireless-capable microscope for biological imaging- Designed specifically for monitoring the neural activity (via genetically encoded calcium indicators) of zebra finches while they sing their courtship songs:
<p align="center" width="100%"> <img width="50%" src="https://github.com/WALIII/Movies/blob/main/SupplimentalVideo02.gif"> </p>You can find the Construction and Assembly Guide for the Microscope, As well as a for the Data Acquisition Box (DAQ). Also, we have a Guide Through a Basic Analysis Pipeline
Wireless Finchscope
The FinchScope is wireless capable and is being used in bats with the Yartsev lab:
<p align="center" width="100%"> <img width="50%" src="https://github.com/WALIII/Movies/blob/main/SupplimentalVideo01.gif"> </p>Additional resources related to this project can be found (here.)https://github.com/WALIII/ImBat
1P2C Microscope (Under Development)
The 1P2C Miniscope is a variant of the FinchScope that incorporates a second excitation path for dual color imaging, or for widefield photo- stimulation. You can find the Construction and Assembly Guide for the Microscope, As well as a for the Data Acquisition Box (DAQ).
Widefield Microscope (Under Development)
The WideField miniature microscope weighs 4g and gives a 4x3mm field of view. You can find the Construction and Assembly Guide for the Microscope, As well as a for the Data Acquisition Box (DAQ).
Active Commutator
The Active Commutator is a low cost, low noise, active (driven by a motor and sensor) electrical rotary joint designed for electrophysiology (single and multichannel micro-electrode arrays) and optophysiology ( optogenetics, miniature microscopes, and fiber photometry).
Referencing
If you use any part of this project in your work, please cite our Journal of Neural Engineering Paper:
Publications that use the FinchScope:
- Liberti, W., et al. Nature Neuroscience 19.12 (2016): 1665-1671.
- Liberti, W., et al. Journal of Neural Engineering 14.4 (2017): 045001.
- Yanny, K., Antipa, N., Liberti, W., et al. (2020). Light: Science & Applications, 9(1), 1-13.
- Cohen, Y, et al. Nature 582.7813 (2020): 539-544.
- Leman, D., et al. (BioRxiv) (2021).
- Singh Alvarado, J., et al. Nature 599.7886 (2021): 635-639.
- Liberti, W., et al. Nature 604.7904 (2022): 98-103.
- Forli, A., et al. Nature 621.7980 (2023): 796-803.
Contributors:
- Will Liberti (U.C. Berkeley, LBNL, Morphosis Neurotech)
- Nathan Perkins (Apple)
- Daniel Leman (Brandeis)
- Jasmine Clevenger (Bosotn U.)
- Will Yen (Boston U.)