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<p align="center"> <a href="https://treeviewer.org"><img src="Icons/Program/Banner.svg"></a> </p>

DOI

Introduction

TreeViewer is a cross-platform program to draw phylogenetic trees. It is based on a "modular" approach, in which small modules are used to perform individual actions (such as computing the coordinates of the nodes of the tree, or drawing the tree branches) that together contribute to the final plot.

Each TreeViewer module has a user manual, and these can be displayed in TreeViewer by opening the Module Manager window (from the Edit menu) or by clicking on the various question mark (?) icons that are shown throughout the program when appropriate.

TreeViewer is written using C# .NET 7 and is available for Windows, macOS (Intel x64 and Apple Silicon ARM) and Linux operating systems. It consists of the main GUI program and a command-line utility that can be used to plot trees that are too large to be previewed on-screen in real time. It is licensed under a GNU Affero GPLv3 license.

Citing TreeViewer

If you use TreeViewer, please cite it as:

Bianchini, G., & Sánchez-Baracaldo, P. (2024).
TreeViewer: Flexible, modular software to visualise and manipulate phylogenetic trees
Ecology and Evolution, 14, e10873. https://doi.org/10.1002/ece3.10873

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Installing TreeViewer

The easiest way to start using TreeViewer is to install the program using the installer for your operating system. Alternatively, you may wish to install the program manually or on a headless server.

Windows

Download TreeViewer-Win-x64.msi and double-click it (if you get a Windows Defender SmartScreen warning, click on More info and choose Run anyway). The installer will guide you through the process, and will do four main things:

  1. Copy the program files (by default in C:\Program Files).
  2. Delete any downloaded modules from previous versions of TreeVIewer.
  3. Add the installation path to the PATH environment variable (so that you can recall TreeViewer from the command line, wherever you are located).
  4. Add a shortcut to TreeViewer to the Start Menu.

Of course, 3 and 4 are optional, and you can decide to skip these steps during the installation.

You can now run TreeViewer using the shortcut that has been created. Alternatively, you can invoke the program from the command line by typing TreeViewer in the command prompt (which you can open by pressing Win+R on your keyboard, typing cmd and pressing Enter). You can also run the command-line version by typing TreeViewerCommandLine.

TreeViewer has been test on Windows 10 and Windows 11 on Intel x64 processors. It seems to work OK on Windows 11 for ARM running in an emulator, but this has not been tested extensively.

macOS

Download TreeViewer-Mac-x64.pkg and double-click it (starting from version 1.2.0, the TreeViewer installer and disk image are fully signed and notarized). If you get a message that the app cannot be opened because it was not downloaded from the App Store, right-click or ctrl-click on the file and choose "Open", then click on the "Open" button in the dialog that opens. The installer will open and guide you through the process. It will do three main things:

  1. Copy the TreeViewer app to the /Applications folder.
  2. Delete any downloaded modules from previous versions of TreeViewer.
  3. Create symlinks to the TreeViewer executables (TreeViewer and TreeViewerCommandLine) in the /usr/local/bin folder.

Once the installer has finished, you can run TreeViewer by opening the App in your Applications folder. You can also run it from the command line by typing TreeViewer (or TreeViewerCommandLine for the command line version) in a terminal window.

TreeViewer has been tested on macOS Catalina (Intel x64), Big Sur (Intel x64), Monterey (Intel x64 and M1 Apple Silicon arm64), Ventura (M1 Apple Silicon arm64), and Sonoma (M1 Apple Silicon arm64).

Linux

TreeViewer has been tested on Debian 12 (bookworm), MX Linux 23.1, Ubuntu 22.04.3, Linux Mint 21.2, openSUSE Leap 15.5, Fedora 38, Manjaro 23.0.4, and CentOS 7. It may work on other distributions as well.

Open a terminal window and download the installer using wget or curl (whichever you prefer/have available):

wget https://github.com/arklumpus/TreeViewer/releases/latest/download/TreeViewer-Linux-x64.run

(To use curl, replace wget with curl -LO). Make the downloaded file executable and execute it as root:

chmod +x TreeViewer-linux-x64.run
sudo "./TreeViewer-linux-x64.run"

Depending on your system, you may have to replace sudo with su -c. You should be prompted for the super-user password. The installer will:

  1. Copy the TreeViewer files to /usr/lib/TreeViewer (this can be changed)
  2. Delete any downloaded modules from previous versions of TreeViewer.
  3. Create symlinks to the TreeViewer and TreeViewerCommandLine executables in /usr/bin (this step can be skipped).
  4. Add TreeViewer to the Desktop menu (this step can be skipped, but it is highly advised not to skip it, unless you are installing TreeViewer on a headless server without a desktop environment).

Note for CentOS users: the more utility on CentOS 7, which is used by the installer to display the licence notice, does not support the -e option. This will result in the licence terms failing to be displayed; you can find the licence document in this repository. This does not otherwise affect the installation.

You can now open TreeViewer using the icon that has been added to the desktop menu, or by typing TreeViewer (or TreeViewerCommandLine for the command-line version) in the command line.

Getting started

The first time you open TreeViewer after installing it, you will be greeted by a welcome window that will allow you to install the latest version of all the available modules. It is advised to install all the available modules, otherwise some actions may not be available in the program.

After this step is completed, on Windows and Linux a new window will open, asking you with which extensions you wish to associate TreeViewer. Choose the files that you would like to open with TreeViewer and click OK to create the file associations (on Windows, you may have to click on the "shield" button to execute this step as an administrator: when you do this, the program will restart after showing an elevation prompt and go straight to the file association window).

On macOS, file associations work differently: the file extensions supported by TreeViewer will automatically be associated with the program, unless you have another program that is already associated with them. You will also need to grant permission to TreeViewer to open files from anywhere on your computer; a window will open with detailed instructions on how to do this.

After these preliminary operations have been completed, the main TreeViewer window should open.

To verify that everything is working correctly, you can download and open the test.tbi file. You should get a warning about the file containing source code: this is a security feature, as files coming from unknown sources may contain malicious code. Whenever you open a file that has not been created by you, you will get a warning like this; however, you can choose to permanently trust the creator of the file, and in this way you will not get prompted again if you open another file that has been created by the same user.

After granting the file permission to load and compile the source code, you should get a plot similar to the one below:

<p align="center"> <img src="test.svg" height="250"> </p>

Troubleshooting and known issues

This section details some known issues that occur on Linux and macOS. In the vast majority of cases, these should not significantly affect your enjoyment of TreeViewer.

Linux

macOS

Manual installation

If you wish to have more control over the installation process, you can manually install TreeViewer following these instructions.

Windows

Download the TreeViewer-Win-x64.zip archive, which contains the binaries and libraries for TreeViewer on Windows. Extract the compressed folder somewhere. You can now start TreeViewer by double-clicking the TreeViewer.exe executable (or TreeViewerCommandLine.exe for the command line version). Note that you need to open the GUI version at least once to set up the modules before you open the command-line version.

If you wish, you can also add the folder where the TreeViewer executables are located to the PATH environment variable:

macOS

Download the TreeViewer-Mac-x64.dmg disk image. Double-click the disk image to mount it (if you get a message that the App cannot be opened because it was not downloaded from the App Store, right-click/ctrl+click it and and choose Open to mount it). Open the TreeViewer disk that should have appeared on your desktop and drag the TreeViewer app to the Applications folder.

When you start TreeViewer for the first time from the icon in your Applications folder, if you get again the message that the app was not downloaded from the App Store you may need to right-click/ctrl-click on it and choose Open. This should only be necessary the first time you open the program; afterwards, you should be able to open TreeViewer normally.

You can also create symlinks to the TreeViewer executables in a folder that is included in your PATH (such as /usr/local/bin): open a terminal and type:

ln -s /Applications/TreeViewer.app/Contents/MacOs/TreeViewer /Applications/TreeViewer.app/Contents/MacOs/TreeViewerCommandLine /usr/local/bin/

This will allow you to run TreeViewer from the command line in any folder.

Linux

Download the TreeViewer-Linux-x64.tar.gz archive and extract it:

wget https://github.com/arklumpus/TreeViewer/releases/latest/download/TreeViewer-Linux-x64.tar.gz
tar -xzf TreeViewer-Linux-x64.tar.gz
rm TreeViewer-Linux-x64.tar.gz

Depending on your system, you may want to replace wget with curl -LO. This will create a folder called TreeViewer-Linux-x64, which contains the TreeViewer executables. You can now run TreeViewer by typing TreeViewer-Linux-x64/TreeViewer.

You can also create symlinks to the TreeViewer executables in a folder that is included in your PATH (such as /usr/bin): open a terminal and type:

ln -s "$(pwd)"/TreeViewer-Linux-x64/TreeViewer "$(pwd)"/TreeViewer-Linux-x64/TreeViewerCommandLine /usr/bin/

You can also add TreeViewer to your desktop menu (this is a prerequisite for creating file associations with TreeViewer) using the following commands:

sed -i "s;@PATHHERE@;$(pwd)/TreeViewer-Linux-x64/TreeViewer;g" TreeViewer-Linux-x64/io.github.arklumpus.TreeViewer.desktop

xdg-icon-resource install --novendor --context apps --size 16 TreeViewer-Linux-x64/Icons/Program-16.png io.github.arklumpus.TreeViewer
xdg-icon-resource install --novendor --context apps --size 32 TreeViewer-Linux-x64/Icons/Program-32.png io.github.arklumpus.TreeViewer
xdg-icon-resource install --novendor --context apps --size 48 TreeViewer-Linux-x64/Icons/Program-48.png io.github.arklumpus.TreeViewer
xdg-icon-resource install --novendor --context apps --size 64 TreeViewer-Linux-x64/Icons/Program-64.png io.github.arklumpus.TreeViewer
xdg-icon-resource install --novendor --context apps --size 256 TreeViewer-Linux-x64/Icons/Program-256.png io.github.arklumpus.TreeViewer
xdg-icon-resource install --novendor --context apps --size 512 TreeViewer-Linux-x64/Icons/Program-512.png io.github.arklumpus.TreeViewer

xdg-desktop-menu install --novendor TreeViewer-Linux-x64/io.github.arklumpus.TreeViewer.desktop

Installing on a headless server without admin privileges

In some instances, you may need to install TreeViewer on a headless server, possibly without admin privileges. To do this, you can start by following the instructions for manual installation (which do not require admin privileges). By doing this, you will have access to the TreeViewer executables, but if you try launching TreeViewerCommandline and enter the command module list available, you will get the following error message:

An error occurred while executing command: module list available
Error message:
  Sequence contains no elements

This is because the modules for TreeViewer are installed the first time that the program is launched from the GUI; if you never launch the GUI (because you are on a headless server), you will not be able to install any modules.

To install all modules from the online repository, you can run the following command:

TreeViewer -I

Alternatively, e.g. if you need to install the moduels on a computer that does not have internet access, you can transfer the modules.tar.gz file to the computer, and then run (note the = sign):

TreeViewer -I=modules.tar.gz

This will install the modules from the specified file, without fetching them from the online repository.

Finally, if you now open TreeViewerCommandLine and enter the module list available command, you should get a list of all the installed modules.

Note that the installed modules and other TreeViewer settings (e.g. key pairs) are always stored in a user-specific directory. On Windows, this is %LOCALAPPDATA%/TreeViewer, while on Linux and macOS it is $HOME/.local/share/TreeViewer. Therefore, even if you install TreeViewer in such a location as to make it accessible to all users on the system, each user will have to run one of the commands above to install the TreeViewer modules.

Compiling TreeViewer from source

To be able to compile TreeViewer from source, you will need to install the .NET 7 SDK for your operating system.

You can use Microsoft Visual Studio to compile the program. The following instructions will cover compiling TreeViewer from the command line, instead.

To fully compile TreeViewer for Windows, macOS and Linux, you will need a computer with Windows 10 or 11 with the Windows Subsystem for Linux installed and a computer with a recent release of macOS.

To create the Windows installer, you will need the WiX toolset, v3.11.2, the scsigntool software, the Windows SDK (which provides the signtool application), as well as a code signing certificate stored on a YubiKey (or another kind of smart card).

First of all, you will need to download the TreeViewer source code: TreeViewer-2.2.0.tar.gz and extract it somewhere on both the Windows machine and the macOS machine.

Then, on the Windows machine, open a command-line window in the folder where you have extracted the source code, and type:

BuildRelease Win-x64
BuildRelease Linux-x64
BuildRelease Mac-x64
BuildRelease Mac-arm64

BuildBinaries-Win-x64 "subject_name" "yubikey_pin"
bash -c ./BuildBinaries-Linux-x64.sh

These commands will compile TreeViewer for all three platforms and create the installers for Windows and Linux, which will be placed in the Binary folder. The arguments for the BuildBinaries-Win-x64 script are the subject name of a code signing certificate and the PIN of the YubiKey/smart card where the certificate is stored. Such a certificate can be obtained (for a fee) from a certificate authority or can be a self-signed certificate.

Now you need to copy the Release/Mac-x64 and Release/Mac-arm64 folders to the corresponding folder on the macOS machine.

To sign the app and the installer on macOS, you will need a "Developer ID Application" certificate and a "Developer ID Installer" certificate, as well as an Apple ID and an app-specific password (which can be generated from the Apple ID page).

On the macOS machine, open a terminal in the folder where you have extracted the TreeViewer source code and type:

./BuildBinaries-Mac-x64.sh "<Developer ID Application Identity>" "<Developer ID Installer Identity>" "<Apple ID>" "<app-specific password>" "<developer team ID>"

Where <Developer ID Application Identity> and <Developer ID Installer Identity> are the names of the certificates in your keychain (e.g. "Developer ID Application: John Smith"). This should create, sign and notarize the installers for macOS in the Binary folder.

Finally, to create the module repository, go back on the Windows machine. You will need a private key file to sign the module files; you can obtain one by running TreeViewer with the --key command line argument. For example, you can use the version of TreeViewer that you have just compiled:

Release\Win-x64\TreeViewer --key ModuleKey

This will create two files called ModuleKey.private.json and ModuleKey.public.json in the current folder.

Note: if your public key is not included in the ModulePublicKeys array in CryptoUtils.cs, trying to install modules from this new module repository will show a warning.

To build the module repository, run:

BuildRepositoryModuleDatabase <private key file>

Where <private key file> is the path to the private key file that you created earlier. This will create the module repository in the Modules folder.