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Quality control of WGS/WES/target alignment data

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Image Qualimap

Description

Perform quality control of WGS/WES/target alignment data.

Dependencies

  1. This pipeline is based on nextflow. As we have several nextflow pipelines, we have centralized the common information in the IARC-nf repository. Please read it carefully as it contains essential information for the installation, basic usage and configuration of nextflow and our pipelines.
  2. samtools: see official installation here. You can avoid installing all the external software by only installing Docker (not available yet). See the IARC-nf repository for more information.)
  3. Qualimap: see official installation here. You can avoid installing all the external software by only installing Docker (not available yet). See the IARC-nf repository for more information.)
  4. Multiqc: see official installation here. You can avoid installing all the external software by only installing Docker (not available yet). See the IARC-nf repository for more information.)

Input

NameDescription
--input_folderFolder containing Fasta files

Parameters

Optional

NameDefault valueDescription
--output_folder.Path to output folder
--qualimap/usr/bin/qualimapPath to Qualimap installation directory
--feature_filemyfeatures.txtQualimap feature file for coverage analysis
--multiqc_confignullconfig yaml file for multiqc
--cpuINTEGERNumber of cpus to be used

Flags

Flags are special parameters without value.

NameDescription
--helpDisplay help

Download test data set

git clone https://github.com/iarcbioinfo/data_test

Usage

nextflow run iarcbioinfo/Qualimap-nf --qualimap /path/to/qualimap --input_folder /path/to/bam --output_folder /path/to/output

Output

NameDescription
HTMLsAn html file for each analysed BAM file, and one containing the aggregated multiQC results

Contributions

NameEmailDescription
Tiffany DelhommeDelhommet@students.iarc.frdeveloper
Maxime ValleeValleem@iarc.frdeveloper
Matthieu FollFollm@iarc.frdeveloper
Nicolas Alcala*AlcalaN@fellows.iarc.frDeveloper to contact for support