Awesome
Quality control of WGS/WES/target alignment data
Description
Perform quality control of WGS/WES/target alignment data.
Dependencies
- This pipeline is based on nextflow. As we have several nextflow pipelines, we have centralized the common information in the IARC-nf repository. Please read it carefully as it contains essential information for the installation, basic usage and configuration of nextflow and our pipelines.
- samtools: see official installation here. You can avoid installing all the external software by only installing Docker (not available yet). See the IARC-nf repository for more information.)
- Qualimap: see official installation here. You can avoid installing all the external software by only installing Docker (not available yet). See the IARC-nf repository for more information.)
- Multiqc: see official installation here. You can avoid installing all the external software by only installing Docker (not available yet). See the IARC-nf repository for more information.)
Input
Name | Description |
---|
--input_folder | Folder containing Fasta files |
Parameters
Optional
Name | Default value | Description |
---|
--output_folder | . | Path to output folder |
--qualimap | /usr/bin/qualimap | Path to Qualimap installation directory |
--feature_file | myfeatures.txt | Qualimap feature file for coverage analysis |
--multiqc_config | null | config yaml file for multiqc |
--cpu | INTEGER | Number of cpus to be used |
Flags
Flags are special parameters without value.
Name | Description |
---|
--help | Display help |
Download test data set
git clone https://github.com/iarcbioinfo/data_test
Usage
nextflow run iarcbioinfo/Qualimap-nf --qualimap /path/to/qualimap --input_folder /path/to/bam --output_folder /path/to/output
Output
Name | Description |
---|
HTMLs | An html file for each analysed BAM file, and one containing the aggregated multiQC results |
Contributions