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Gene Prediction with AUGUSTUS

INTRODUCTION
INSTALLATION
RUNNING AUGUSTUS
WEB-SERVER
COMPARATIVE GENE PREDICTION
AUTHORS AND CONTACT
REFERENCES
LICENSES

INTRODUCTION

AUGUSTUS is a program to find genes and their structures in one or more genomes. More ...

INSTALLATION

Windows

Windows users can use the Windows Subsystem for Linux (WSL) to install AUGUSTUS exactly as described below for Linux. How to set up the WSL for AUGUSTUS is described here.

Ubuntu 18.04, Debian 9 or later

Until Ubuntu 21.04 and Debian 11 only as single-genome version, since then with capability for comparative gene prediction.

sudo apt install augustus augustus-data augustus-doc

Docker

Create a docker image from Dockerfile using:

git clone https://github.com/Gaius-Augustus/Augustus.git
cd Augustus
docker build -t augustus .

Singularity

Create a Singularity Image File from the Singularity Definition File using

git clone https://github.com/Gaius-Augustus/Augustus.git
cd Augustus
singularity build augustus.sif Singularity.def

Building AUGUSTUS from source

See INSTALL.md for details.

Download source code from github and compile:

git clone https://github.com/Gaius-Augustus/Augustus.git
cd Augustus
make augustus

After compilation has finished, the command bin/augustus should be executable and print a usage message.

For utilities use

make auxprogs

Install locally

As a normal user, add the directory of the executables to the PATH environment variable, for example:

export PATH=~/augustus/bin:~/augustus/scripts:$PATH

Install globally

You can install AUGUSTUS globally, if you have root privileges, for example:

sudo make install

Alternatively, you can exectue similar commands to those in the "install" section of the top-level Makefile to customize the global installation.

Optional: set environment variable AUGUSTUS_CONFIG_PATH

If the environment variable AUGUSTUS_CONFIG_PATH is set, augustus and etraining will look there for the config directory that contains the configuration and parameter files, e.g. '~/augustus/config'. You may want to add this line to a startup script (like ~/.bashrc).

export AUGUSTUS_CONFIG_PATH=/my_path_to_AUGUSTUS/augustus/config/

If this environment variable is not set, then the programs will look in the path ../config relative to the directory in which the executable lies. As a third alternative, you can specify this directory on the command line when you run augustus: --AUGUSTUS_CONFIG_PATH=/my_path_to_AUGUSTUS/augustus/config/

WEB-SERVER

AUGUSTUS can also be run through a web-interface at http://bioinf.uni-greifswald.de/augustus/ and a web service at http://bioinf.uni-greifswald.de/webaugustus/.

REFERENCES AND DOCUMENTATION

Mario Stanke, Mark Diekhans, Robert Baertsch, David Haussler (2008). Using native and syntenically mapped cDNA alignments to improve de novo gene finding. Bioinformatics, 24(5), pages 637–644, doi: 10.1093/bioinformatics/btn013

For further references see docs/REFERENCES.md

3 book chapters with command line walkthroughs

LICENSES

All source code, i.e.

• the AUGUSTUS source code (src/*.cc, include/*.hh)
• the scripts (scripts/*)
• the auxiliary programs (auxprogs/)
• the tree-parser (src/scanner, src/parser)
• the unit tests (src/unittests)

is under the Artistic License.